A technique for the measurement of renal ATP in a large animal model of septic shock

Abstract

Background: The mechanisms responsible for acute renal failure in sepsis are not understood. Measurement of tissue ATP might help to understand this process but, in the large animal, it is hampered by major technical difficulties. Objective: To develop a technique to monitor ATP in the kidney of a large mammal during the induction of septic shock and then circulatory arrest. Methods: Implantation of a custom-made phosphorus coil around the left kidney. Induction of septic shock by intravenous E. coli administration. Acquisition of 31P magnetic resonance (MR) spectroscopic data at 3-tesla before and during septic shock over several hours. Induction of euthanasia and measurement of the same 31..