Journal article
Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
MR Bogwitz, H Chung, L Magoc, S Rigby, W Wong, M O'Keefe, JA McKenzie, P Batterham, PJ Daborn
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2005
Abstract
Lufenuron is an insect growth regulator insecticide mainly used for the control of the cat flea. To understand mechanisms of resistance to lufenuron, we have characterized lufenuron resistance in a natural population of Drosophila melanogaster. In this study we have used precise genetic mapping to identify a mechanism of lufenuron resistance: the overexpression of the cytochrome P450 gene Cyp12a4. Cyp12a4 is predicted to encode a mitochondrial cytochrome P450 enzyme. Expression of Cyp12a4 in D. melanogaster third-instar larvae was detected in the midgut and Malpighian tubules of both lufenuron-resistant and wild-type strains. The level of Cyp12a4 expression in the midgut is higher in the luf..
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