Journal article

The use of pimonidazole to characterise hypoxia in the internal environment of an in vivo tissue engineering chamber

SOP Hofer, GM Mitchell, AJ Penington, WA Morrison, R RomeoMeeuw, E Keramidaris, J Palmer, KR Knight

British Journal of Plastic Surgery | CHURCHILL LIVINGSTONE | Published : 2005

Abstract

UNLABELLED: The distribution of hypoxic cells in an in vivo tissue engineering chamber was investigated up to 28 days post-implantation. METHODS: Arteriovenous loops were constructed and placed into bi-valved polycarbonate chambers containing 2 x 10(6) rat fibroblasts in basement membrane gel (BM gel). Chambers were inserted subcutaneously in the groin of male rats and harvested at 3 (n = 6), 7 (n = 6), 14 (n = 4) or 28 (n = 4) days. Ninety minutes before harvest, pimonidazole (60 mg/kg) was injected intraperitoneally. Chamber tissue was removed, immersion fixed, paraffin embedded, sectioned and stained immunohistochemically using hypoxyprobe-1 Mab that detects reduced pimonidazole adducts f..

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