Journal article

T cell determinants incorporating beta-amino acid residues are protease resistant and remain immunogenic in vivo

AI Webb, MA Dunstone, NA Williamson, JD Price, A de Kauwe, WS Chen, A Oakley, P Perlmutter, J McCluskey, MI Aguilar, J Rossjohn, AW Purcell

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2005

Abstract

A major hurdle in designing successful epitope-based vaccines resides in the delivery, stability, and immunogenicity of the peptide immunogen. The short-lived nature of unmodified peptide-based vaccines in vivo limits their therapeutic application in the immunotherapy of cancers and chronic viral infections as well as their use in generating prophylactic immunity. The incorporation of beta-amino acids into peptides decreases proteolysis, yet its potential application in the rational design of T cell mimotopes is poorly understood. To address this, we have replaced each residue of the SIINFEKL epitope individually with the corresponding beta-amino acid and examined the resultant efficacy of t..

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