Regulation of Toll-like receptor (TLR)2 and TLR4 on CD14 dimCD16 monocytes in response to sepsis-related antigens

Abstract

Rapid overproduction of proinflammatory cytokines are characteristic of sepsis. CD14dimCD16+ monocytes are thought to be major producers of cytokine and have been shown to be elevated in septic patients. Toll-like receptors (TLR) are pattern recognition receptors important in mediating the innate immune response and their activation can lead to production of cytokines. Using whole blood culture and flow cytometry we have investigated TLR2 and TLR4 regulation after stimulation with sepsis-relevant antigens [lipopolysaccharide (LPS), Staphylococcal enterotoxin B (SEB) and peptidoglycan (PGN)]. The percentage of CD14dimCD16+ monocyte population expanded at 20 h post-stimulation, after a rise in..