Journal article

Homogeneous, competitive fluorescence quenching immunoassay based on gold nanoparticle/polyelectrolyte coated latex particles

N Kato, F Caruso

Journal of Physical Chemistry B | AMER CHEMICAL SOC | Published : 2005

Abstract

This study reports a homogeneous and competitive fluorescence quenching immunoassay based on gold nanoparticle/polyelectrolyte (Au(NP)/PE) coated latex particles prepared by the layer-by-layer (LbL) technique. First, the resonant energy transfer from a layer of fluorescent PEs to Au(NP) in LbL assembled films on planar substrates was investigated. The quenching efficiency (QE) for the planar films depended on the cube of the distance between the two layers. A QE of 50% was achieved at a distance of ca. 15 nm, indicating that the Au(NP)/PE system is suitable for detecting binding/release events for antibodies. A homogeneous, competitive binding immunoassay for biotin was designed based on Au(..

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University of Melbourne Researchers