Journal article

Recombinant shark natural antibodies to thyroglobulin

SF Schluter, I Jensen, PA Ramsland, JJ Marchalonis

JOURNAL OF MOLECULAR RECOGNITION | WILEY | Published : 2005

Abstract

As cartilaginous fish are the vertebrates most distal from man to produce antibodies, fundamental information regarding conservation and variation of the antigen binding site should be gained by comparing the properties of antibodies directed against the same antigen from the two species. Since monoclonal cell lines cannot be generated using shark B cells, we isolated antigen binding recombinant single chain Fv antibodies (scFv) comprising of the complete variable regions from shark light and heavy chains. Thyroglobulin was used as the selecting antigen as both sharks and humans express natural antibodies to mammalian thyroglobulin in the absence of purposeful immunization. We report that re..

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University of Melbourne Researchers