Enzyme multilayer-modified porous membranes as biocatalysts

Abstract

Porous polycarbonate (PC) membranes with pore diameters of either 400 or 100 nm were used as supports for the layer-by-layer deposition of peroxidase-poly(sodium 4-styrenesulfonate) complexes [(POD-PSS)C] and oppositely charged poly(allylamine hydrochloride) (PAH) to prepare high-surface-area thin films for biocatalysis. Formation of the multilayer films was verified by scanning electron microscopy and transmission electron microscopy following dissolution of the porous PC template to generate POD/polyelectrolyte (PE) tubes. An average thickness of ∼5 nm was calculated for each (POD-PSS)C/PAH bilayer, as determined from microscopy images. The activity of the POD/PE multilayer films was found..