Journal article

The immunoquantification of caveolin-1 and eNOS in human and rabbit diseased blood vessels

A Zulli, BF Buxton, MJ Black, ZQ Ming, A Cameron, DL Hare

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | HISTOCHEMICAL SOC INC | Published : 2006

Abstract

In this study, caveolin-1 (cav-1), an inhibitor of endothelial nitric oxide synthase (eNOS), was semi-quantified in diseased human and rabbit blood vessels. New Zealand White rabbits were fed, for 12 weeks, a high methionine diet (to induce intimal hyperplasia), 0.5% cholesterol diet, a normal diet, or the combination of both experimental diets. Excess segments of human internal mammary arteries (IMA) and radial arteries (RA) were obtained from patients undergoing coronary artery bypass surgery. eNOS and cav-1 were localized throughout both human and rabbit vessels. In rabbit arteries, eNOS was significantly increased in the endothelium overlying intimal thickening and atherosclerotic plaque..

View full abstract