Journal article

The pluripotency homeobox gene NANOG is expressed in human germ cell tumors

AH Hart, L Hartley, K Parker, M Ibrahim, LHJ Looijenga, M Pauchnik, CW Chow, L Robb

CANCER | JOHN WILEY & SONS INC | Published : 2005

Abstract

BACKGROUND: The NANOG gene, a member of the homeobox family of DNA binding transcription factors, was recently identified in a screen for pluripotency-promoting genes. NANOG overexpression in murine embryonic stem cells is sufficient to maintain self-renewal and to block differentiation. The NANOG gene is located on human chromosome 12p13, a region frequently duplicated in human tumors of germ cell origin and in cultured human embryonic stem cells. Here we investigate the expression and gene copy number of NANOG in human germ cells and tumors of germ cell origin. METHODS: Immunohistochemistry and quantitative polymerase chain reaction (QPCR) were used to examine the expression and gene copy ..

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