Conserved expression of a novel gene during gonadal development

Abstract

We isolated the novel gene Gonad Expressed Transcript (GET) from a chicken embryonic gonad library enriched for differentially expressed male transcripts. Chicken GET encodes a predicted protein containing a Pfam-B 30624 domain with homology to a putative orthologue in mammals. Chicken GET expression was confined to the developing urogenital system. It was first detected in the glomerulus of the mesonephros of both sexes from embryonic day (E) 2.5. At E4.5, expression switches to the gonad of both sexes and then localizes to the gonadal cortex. We isolated the putative mouse orthologue and examined expression in the mouse embryo. Gonadal expression was conserved. Ovarian expression localized..