Journal article

Meiotic and epigenetic defects in Dnmt3L-knockout mouse spermatogenesis

KE Webster, MK O'Bryan, S Fletcher, PE Crewther, U Aapola, J Craig, DK Harrison, H Aung, N Phutikanit, R Lyle, SJ Meachem, SE Antonarakis, DM de Krester, MP Hedger, P Peterson, BJ Carroll, HS Scott

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2005

Abstract

The production of mature germ cells capable of generating totipotent zygotes is a highly specialized and sexually dimorphic process. The transition from diploid primordial germ cell to haploid spermatozoa requires genome-wide reprogramming of DNA methylation, stage- and testis-specific gene expression, mitotic and meiotic division, and the histone-protamine transition, all requiring unique epigenetic control. Dnmt3L, a DNA methyltransferase regulator, is expressed during gametogenesis, and its deletion results in sterility. We found that during spermatogenesis, Dnmt3L contributes to the acquisition of DNA methylation at paternally imprinted regions, unique nonpericentric heterochromatic sequ..

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