Agrobacterium-mediated transformation and generation of male sterile lines of Australian canola

Abstract

An efficient protocol for Agrobacterium-mediated transformation of Australian commercial canola cultivars using seedling explants is described. Seedling explants provide flexibility and reduction in labour and maintenance costs of explant sources. Five commercial genotypes of canola were successfully transformed using the developed protocol. A transformation efficiency of 67% was obtained for genotypes Oscar and RK7 from cotyledon explants, which was higher than the rate for the most commonly used cultivar Westar (33%). Comparison of different seedling explants showed that although transgenic plants could be regenerated from all explant types (cotyledons, hypocotyls, and roots) used, the num..