Journal article

Opposing roles of endothelial and smooth muscle phosphatidylinositol 3-kinase in vasoconstriction: Effects of rho-kinase and hypertension

K Budzyn, PD Marley, CG Sobey

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS | AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS | Published : 2005

Abstract

Phosphatidylinositol 3-kinase (PI3K) can activate endothelial nitric oxide synthase (eNOS), leading to production of the vasodilator NO. In contrast, vascular smooth muscle (VSM) PI3K may partially mediate vascular contraction, particularly during hypertension. We tested whether endothelial and VSM PI3K may have opposing functional roles in regulating vascular contraction. Secondly, we tested whether the procontractile protein rho-kinase can suppress endothelial PI3K/eNOS activity in intact arteries, thus contributing to vasoconstriction by G protein-coupled receptor (GPCR) agonists. We studied contractile responses to the GPCR agonist phenylephrine, and the receptor-independent vasoconstric..

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University of Melbourne Researchers