M-CSF induces the stable interaction of cFms with αVβ3 integrin in osteoclasts

Abstract

The macrophage colony stimulating factor receptor (cFms) and αVβ3 integrin are both abundantly expressed and play critical roles in the differentiation, survival and migration of osteoclasts. We have previously demonstrated that cross-talk between cFms- and αVβ3-mediated signaling pathways regulated the cytoskeletal organization required for osteoclast migration. To investigate the nature of interaction between the two receptors, we sequentially used anion-exchange chromatography and immunoprecipitation to purify αVβ3-associated protein complexes. We have demonstrated that cFms stably associated with αVβ3 in osteoclasts during adhesion, and that the association was induced by macrophage colo..