Proteomic analysis of mouse growth plate cartilage
Daniele Belluoccio, Richard Wilson, David J Thornton, Tristan P Wallis, Jeffrey J Gorman, John F Bateman
PROTEOMICS | WILEY-V C H VERLAG GMBH | Published : 2006
Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.