Journal article

Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix

Aditya V Vashi, Keren M Abberton, Gregory P Thomas, Wayne A Morrison, Andrea J O'Connor, Justin J Cooper-White, Erik W Thompson

TISSUE ENGINEERING | MARY ANN LIEBERT, INC | Published : 2006

Abstract

Adipose tissue forms when basement membrane extract (Matrigel) and fibroblast growth factor-2 (FGF-2) are added to our mouse tissue engineering chamber model. A mouse tumor extract, Matrigel is unsuitable for human clinical application, and finding an alternative to Matrigel is essential. In this study we generated adipose tissue in the chamber model without using Matrigel by controlled release of FGF-2 in a type I collagen matrix. FGF-2 was impregnated into biodegradable gelatin microspheres for its slow release. The chambers were filled with these microspheres suspended in 60 microL collagen gel. Injection of collagen containing free FGF-2 or collagen containing gelatin microspheres with b..

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