Journal article

Impaired "outside-in" integrin alpha(IIb)beta(3) signaling and thrombus stability in TSSC6-deficient mice

Matt W Goschnick, Lai-Man Lau, Janet L Wee, Yong S Liu, P Mark Hogarth, Lorraine M Robb, Michael J Hickey, Mark D Wright, Denise E Jackson

BLOOD | AMER SOC HEMATOLOGY | Published : 2006

Abstract

We investigated the role of the hematopoietic-specific tetraspanin superfamily member, TSSC6, in platelet function using wild-type mice and TSSC6-deficient mice. TSSC6 is expressed on the surface of murine platelets and is up-regulated by thrombin stimulation, indicating an intracellular pool of TSSC6. Immunoprecipitation/Western blot studies reveal a constitutive physical association of TSSC6 with the integrin alpha(IIb)beta(3) complex under strong detergent conditions. In vivo evaluation of hemostasis by tail bleeding revealed increased bleeding time, volume of blood lost, and evidence of tail rebleeds in TSSC6 null mice, indicating unstable hemostasis. Using ex vivo techniques, we showed ..

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