Journal article

Ubiquitin-conjugating enzyme genes in Oesophagostomum dentatum

Pauline A Cottee, Youssef G Abs El-Osta, Alasdair J Nisbet, Robin B Gasser

PARASITOLOGY RESEARCH | SPRINGER | Published : 2006

Abstract

Full-length genes representing different isoforms of the ubiquitin-conjugating enzyme UBC-2 were isolated from Oesophagostomum dentatum, cloned and sequenced. The alignment of their sequences (designated Od-ubc-2.1 to Od-ubc-2.3) revealed nucleotide variation at three positions within the predicted open reading frame of 444 bp. Substitutions were at positions 141 (AG), 142 (AG) and 296 (TC). Both former substitutions resulted in amino acid changes from a glycine residue to an arginine residue, whereas the latter resulted in a change from isoleucine to threonine. Comparison of predicted OD-UBC-2 with UBC-2 (protein) homologues/orthologues from 12 other species representing nematodes, Drosophi..

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