Journal article

Haemonchus contortus: Prokaryotic expression and enzyme activity of recombinant HcSTK, a serine/threonine protein kinase

Sia Nikolaou, Dadna Hartman, Alasdair J Nisbet, Robin B Gasser

EXPERIMENTAL PARASITOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2006

Abstract

Members of the PAR-1/MARK serine/threonine protein kinase (STK) subfamily are important regulators of the cytoskeleton, and their characterization can provide insights into a number of critical processes relating to the development and survival of an organism. We previously investigated the mRNA expression for and organization of a gene (hcstk) representing HcSTK, an STK from the parasitic nematode Haemonchus contortus. In the present study, a recombinant form of HcSTK was expressed and characterized. Affinity-purified anti-HcSTK antibodies reacted with native HcSTK in protein homogenates extracted from third-stage larvae (L3) of H. contortus and were also used to immunolocalize the protein ..

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University of Melbourne Researchers