Journal article

Chronic exposure to intra-amniotic lipopolysaccharide affects the ovine fetal brain

Ilias Nitsos, Sandra M Rees, Jhodie Duncan, Boris W Kramer, Richard Harding, John P Newnham, Timothy JM Moss

JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION | ELSEVIER SCIENCE INC | Published : 2006

Abstract

OBJECTIVE: Fetal brain injury is associated with chorioamnionitis, which is often present without signs of overt infection or fetal compromise. We aimed to determine if prolonged exposure to intrauterine inflammation caused by intra-amniotic infusion of lipopolysaccharide (LPS) would affect the fetal brain. METHODS: At 80 days of pregnancy ewes bearing singletons had osmotic pumps implanted intra-amniotically to infuse Escherichia coli LPS (055:B5; n = 8) or saline (n = 7) for 28 days. At delivery (110 days), umbilical arterial blood and chorioamnion were assessed for inflammation; cytokine concentrations (interleukin [IL]-6 and IL-8) in amniotic fluid and fetal and maternal plasma were meas..

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