Journal article

The conformation of acetylated virginiamycin M-1 and virginiamycin M-1 in explicit solvents

Chai Ann Ng, Wen Zhao, Jason Dang, Mikael Bergdahl, Frances Separovic, Robert TC Brownlee, Robert P Metzger

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | ELSEVIER SCIENCE BV | Published : 2007

Abstract

The three-dimensional structure of acetylated virginiamycin M(1) (acetylated VM1) in chloroform and in a water/acetonitrile mixture (83:17 v/v) have been established through 2D high resolution NMR experiments and molecular dynamics modeling and the results compared with the conformation of the antibiotic VM1 in the same and other solvents. The results indicated that acetylation of the C-14 OH group of VM1 caused it to rotate about 90 degrees from the position it assumed in non-acetylated VM1. The conformation of both VM1 and acetylated VM1 appear to flatten in moving from a nonpolar to polar solvent. However, the acetylated form has a more hydrophobic nature. The acetylated VM1 in chloroform..

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University of Melbourne Researchers