Journal article
In vivo quantification of fat content in mice using the Hologic QDR 4500A densitometer
SM Senn, S Kantor, BJ Leury, S Andrikopoulos, TJ O'Brien, MJ Morris, J Proietto, JD Wark
Obesity Research and Clinical Practice | Published : 2007
Abstract
Purpose: Validation of dual-energy X-ray absorptiometry (DXA) with the Hologic QDR 4500A (QDR 4500) Fan Beam X-ray densitometer for in vivo assessment of body fat content in mice. Methods: Precision of DXA fat measurement was assessed by repeated in vivo scanning and re-positioning of different sized mice (17.6, 24.6, 34.2 g). DXA fat and total mass measurements were correlated with dissected tissue weights in 240 female adult mice of seven strains (mean weights 21.9-26.8 g). Accuracy of DXA fat tissue measurements was assessed by chemical analysis in a subgroup of 40 female decapitated mice (mean weights 19.6-28.4 g). Results: Precision of the DXA measurements for fat mass was dependent on ..
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Funding Acknowledgements
The authors are grateful for the assistance of the Department of Medicine and Bone and Mineral Service, The Royal Melbourne Hospital, to Trang Nguyen for her assistance with the chemical analysis experiments and to Dr. Damian Myers, Department of Medicine, for his collaboration on this project. They would also like to acknowledge Sanofi-Synthelabo for partly funding this research.