Conference Proceedings
Two-photon characterization and microscopy of porphyrin photosensitisers
S Mathai, DK Bird, SS Stylli, TA Smith, KP Ghiggino
Progress in Biomedical Optics and Imaging Proceedings of SPIE | Published : 2007
DOI: 10.1117/12.707559
Abstract
The benefits of two-photon fluorescence microscopy of biological samples are vast arising from the utilisation of low energy light. The two-photon absorption cross sections (02) of the di-cation free-base and metallated forms of hematoporphyrin derivative (HpD), hematoporphyrin IX (Hp9) and a boronated protoporphyrin (BOPP) are obtained to ascertain their effectiveness as fluorophores for use in two-photon microscopy. The open-aperture Z-scan and the two-photon induced fluorescence (TPIF) techniques, each capable of providing information regarding the nonlinear absorption, are employed to determine σ2 of the various porphyrins at an excitation wavelength of 800 nm. A significant disparity in..
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