Journal article
Glycosynthases: Mutant glycosidases for glycoside synthesis
SJ Williams, SG Withers
AUSTRALIAN JOURNAL OF CHEMISTRY | C S I R O PUBLISHING | Published : 2002
DOI: 10.1071/CH02005
Abstract
Glycosynthases are engineered mutant glycosidases that catalyse the formation of a glycosidic bond from a glycosyl donor and an acceptor alcohol. They are constructed by mutation of the enzymic nucleophile of a retaining glycosidase to a small non-nucleophilic residue. To date, five glycosynthases have been reported capable of synthesizing a range of β-glycosidic linkages. Methods to integrate protecting groups into glycosynthase-mediated glycosylations have been developed that broaden their applicability and enable finer control over product formation. Mutagenesis studies have improved the catalytic power of the original Abg glycosynthase, and a general methodology has been developed that a..
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