Journal article

Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae

R Chopra-Dewasthaly, M Marenda, R Rosengarten, W Jechlinger, C Citti

FEMS MICROBIOLOGY LETTERS | OXFORD UNIV PRESS | Published : 2005

Abstract

Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic tools has been a major impediment in studying the pathogenic mechanisms of M. agalactiae. This study describes the identification and cloning of the M. agalactiae origin of replication (oriC) in order to construct the first shuttle vectors for targeted gene disruption, gene complementation and expression studies. Additionally, this report provides the first evidence of the occurren..

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University of Melbourne Researchers