Journal article

Cloning, expression and biological activity of equine interleukin (IL)-5

FM Cunningham, E Vandergrifft, SR Bailey, MF Sepulveda, NT Goode, DW Horohov

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | ELSEVIER SCIENCE BV | Published : 2003

Abstract

The cytokine, interleukin (IL)-5 stimulates eosinophil differentiation, activation and survival and can prime these cells, increasing the response to other mediators. In view of its many effects on eosinophils, IL-5 has been implicated in the pathogenesis of allergic disease in man. Here we report the cloning of equine IL-5 and expression of the recombinant protein by transfection of Chinese hamster ovary (CHO) cells. The cloned cDNA sequence consisted of 405 nucleotides and encoded a protein of 135 amino acids. There is >85% identity with feline, bovine, ovine, canine, and human IL-5 sequences at the nucleotide and protein level. Supernatants containing equine IL-5 were also examined for bi..

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University of Melbourne Researchers