Adherence of eosinophils from allergic and normal ponies to cultured equine endothelial cells

Abstract

Objectives: To compare adherence of stimulated and unstimulated eosinophils from allergic and normal ponies to cultured equine vascular endothelial cells (equine digital vein endothelial cells; EDVEC) and examine the effect of eosinophil-derived factor(s) on cell adherence. Methods: Eosinophil adherence to unstimulated EDVEC or EDVEC pretreated with IL-1β or supernatants from stimulated eosinophils was measured. Supernatants were also assayed for TNFα and IL-1β-like bioactivity. Results: Adherence of unstimulated and rhIL-5 (10 ng/ml)-stimulated eosinophils from allergic ponies to rhIL-1βtreated EDVEC was significantly greater than that of cells from normal ponies. Pretreatment of EDVEC with..