Journal article

Antisense repression of cytosolic phosphoglucomutase in potato (Solanum tuberosum) results in severe growth retardation, reduction in tuber number and altered carbon metabolism

AR Fernie, E Tauberger, A Lytovchenko, U Roessner, L Willmitzer, RN Trethewey

PLANTA | SPRINGER | Published : 2002


The aim of this work was to investigate the role of cytosolic phosphoglucomutase (PGM; EC in the regulation of carbohydrate metabolism. Many in vitro studies have indicated that PGM plays a central role in carbohydrate metabolism; however, until now the importance of this enzyme in plants has not been subject to reverse-genetics investigations. With this intention we cloned the cytosolic isoform of potato PGM (StcPGM) and expressed this in the antisense orientation under the control of the CaMV 35 S promoter in potato plants. We confirmed that these plants contained reduced total PGM activity and that loss in activity was due specifically to a reduction in cytosolic PGM activity. Th..

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University of Melbourne Researchers