Journal article

Spatial and temporal gene expression profiling of the contused rat spinal cord

JB Aimone, JL Leasure, VM Perreau, M Thallmair

EXPERIMENTAL NEUROLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2004

Abstract

Microarray technology was used to examine gene expression changes following contusive injury of the adult rat spinal cord. To obtain a global understanding of the changes triggered by the injury, differential gene expression was examined spatially, using tissue samples from the epicenter of injury as well as 1 cm rostral and 1 cm caudal to the epicenter, and temporally, at 3 h, 24 h, 7 days, and 35 days post-injury. To filter out gene expression changes that were due to the laminectomy, samples of contused tissue were compared to laminectomy-only controls. We took advantage of four different, complementary methods of data analysis to detect differentially expressed genes. We have identified ..

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