Journal article

Acetaldehyde mediates growth stimulation of ethanol-stressed Saccharomyces cerevisiae: evidence of a redox-driven mechanism

Frank Vriesekoop, Andrew R Barber, Neville B Pamment



The ability of acetaldehyde (90 mg l(-1)) to stimulate ethanol-stressed S. cerevisiae fermentations is examined and reasons for the effect explored. Alternative metabolic electron acceptors generated similar stimulatory effects to acetaldehyde, decreasing the ethanol-induced growth lag phase from 9 h to 3 h, suggesting a redox-driven effect. The exposure to ethanol caused an instant 60% decline in intracellular NAD(+) which was largely prevented by the addition of acetaldehyde. Furthermore, the exposure to ethanol affected glycolysis by decreasing the rate of glucose utilisation from 0.33 g glucose g(-1) biomass h(-1) to 0.11 g glucose g(-1) biomass h(-1), while the addition of acetaldehyde ..

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University of Melbourne Researchers