Journal article

Layer-by-layer construction of novel biofunctional fluorescent microparticles for immunoassay applications

WJ Yang, D Trau, R Renneberg, NT Yu, F Caruso

JOURNAL OF COLLOID AND INTERFACE SCIENCE | ACADEMIC PRESS INC | Published : 2001

Abstract

A novel class of biofunctional fluorescent microparticles for application in immunoassays was constructed by using the layer-by-layer self-assembly method to deposit multiple layers of fluorescently labeled polyelectrolytes onto colloidal particles, followed by deposition of a protein (immunoglobulin G, IgG) layer. Microelectrophoresis experiments revealed alternating negative and positive zeta-potentials with deposition of each successive polyelectrolyte layer, indicating that the alternate electrostatic adsorption of polyelectrolytes of opposite charge was successfully achieved. Transmission electron microscopy images showed a change of the particle surface texture after polyelectrolyte mu..

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University of Melbourne Researchers