Journal article

Common origins of MDA-MB-435 cells from various sources with those shown to have melonoma properties

JM Rae, SJ Ramus, M Waltham, JE Armes, IG Campbell, R Clarke, RJ Barndt, MD Johnson, EW Thompson

CLINICAL & EXPERIMENTAL METASTASIS | SPRINGER | Published : 2004

Abstract

Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). G..

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