Journal article

Optimizing microarray in experimental hypertension

MF Shannon, KUS McKenzie, A Edgley, S Rao, K Peng, A Shweta, CG Schyvens, WP Anderson, SR Wilson, YE Pittelkow, S Ohms, JA Whitworth

KIDNEY INTERNATIONAL | ELSEVIER SCIENCE INC | Published : 2005

Abstract

BACKGROUND: Genetic noise between outbred animals can potentially be a major confounder in the use of microarray technology for gene expression profiling. The study of paired organs from the same animal offers an alternative approach (e.g., for studies of the kidney in experimental hypertension). The present study was undertaken to determine the level of genetic noise between outbred adult Sprague-Dawley (SD) rats, and to determine the effects of unilateral nephrectomy on changes in gene expression as a basis for the design of microarray studies in experimental hypertension. METHODS: Male SD rats (approximately 130 g) were acclimatized before measurement of tail-cuff systolic blood pressure ..

View full abstract