Journal article

Proteomic analysis and characterisation of human cervico-vaginal fluid proteins

Megan KW Di Quinzio, Karen Oliva, Sarah J Holdsworth, Mustafa Ayhan, Susan P Walker, Gregory E Rice, Harry M Georgiou, Michael Permezel

AUSTRALIAN & NEW ZEALAND JOURNAL OF OBSTETRICS & GYNAECOLOGY | WILEY | Published : 2007

Abstract

AIM: Cervico-vaginal fluid (CVF) may provide insight into the biochemical pathways of human reproduction and parturition. The aim of this study was to establish a 2-D electrophoretic map of human CVF in healthy, pregnant women at term. METHODS: CVF was collected, concentrated and processed by routine 2-D polyacrylamide gel electrophoresis using pH 4-7-immobilised pH gradient strips and 8-16% gradient polyacrylamide gels. Imaged gels were analysed, yielding more than 400 proteins. A total of 157 proteins were common to all gels with a subgroup of the most abundant proteins being excised and characterised either by MALDI or by electrospray ion-trap mass spectrometry. RESULTS: Twenty-one protei..

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