Journal article

Induction of epithelial to mesenchymal transition in PMC42-LA human breast carcinoma cells by carcinoma-associated fibroblast secreted factors

Stephanie C Lebret, Donald F Newgreen, Erik W Thompson, M Leigh Ackland

BREAST CANCER RESEARCH | BMC | Published : 2007

Abstract

BACKGROUND: Breast carcinoma is accompanied by changes in the acellular and cellular components of the microenvironment, the latter typified by a switch from fibroblasts to myofibroblasts. METHODS: We utilised conditioned media cultures, Western blot analysis and immunocytochemistry to investigate the differential effects of normal mammary fibroblasts (NMFs) and mammary cancer-associated fibroblasts (CAFs) on the phenotype and behaviour of PMC42-LA breast cancer cells. NMFs were obtained from a mammary gland at reduction mammoplasty, and CAFs from a mammary carcinoma after resection. RESULTS: We found greater expression of myofibroblastic markers in CAFs than in NMFs. Medium from both CAFs a..

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