Journal article

Host rather than graft origin of matrigel-induced adipose tissue in the murine tissue-engineering chamber

Filip Stillaert, Michael Findlay, Jason Palmer, Rejhan Idrizi, Shirley Cheang, Aurora Messina, Keren Abberton, Wayne Morrison, Erik W Thompson

TISSUE ENGINEERING | MARY ANN LIEBERT, INC | Published : 2007

Abstract

We have recently shown that Matrigel-filled chambers containing fibroblast growth factor-2 (FGF2) and placed around an epigastric pedicle in the mouse were highly adipogenic. Contact of this construct with pre-existing tissue or a free adipose graft was required. To further investigate the mechanisms underpinning formation of new adipose tissue, we seeded these chambers with human adipose biopsies and human adipose-derived cell populations in severe combined immunodeficient mice and assessed the origin of the resultant adipose tissue after 6 weeks using species-specific probes. The tissues were negative for human-specific vimentin labeling, suggesting that the fat originates from the murine ..

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