Journal article

Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing

Min Hu, Aaron R Jex, Bronwyn E Campbell, Robin B Gasser

NATURE PROTOCOLS | NATURE PUBLISHING GROUP | Published : 2007

Abstract

Exploring mitochondrial (mt) genomes has significant implications for various fundamental research areas, including mt biochemistry and physiology, and, importantly, such genomes provide a rich source of markers for population genetics and systematic studies. Although some progress has been made, there is a paucity of information on mt genomes for many metazoan organisms, particularly invertebrates such as parasitic helminths, which relates mainly to the technical limitations associated with sequencing from tiny amounts of material. In this article, we describe a practical long PCR approach for the amplification and subsequent sequencing of the entire mt genome from individual helminths, whi..

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