Journal article

PCR approach for the detection of Trypanosoma brucei and T-equiperdum and their differentiation from T-evansi based on maxicircle kinetoplast DNA

Feng-Jun Li, Robin B Gasser, De-Hua Lai, Filip Claes, Xing-Quan Zhu, Zhao-Rong Lun

MOLECULAR AND CELLULAR PROBES | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2007

Abstract

The goal of this study was to develop a PCR approach based on the sequence of maxicircle kinetoplast DNA (kDNA) of Trypanosoma brucei to distinguish T. brucei/T. equiperdum from T. evansi and to evaluate its diagnostic use for their detection in blood samples. Primers derived from the sequence of the maxicircle kDNA of T. brucei, encoding the NADH dehydrogenase subunit 5 (nad5) gene, were used to test the PCR-amplification from T. brucei (including T. b. brucei and T. b. rhodesiense), T. equiperdum, T. evansi, T. vivax and T. congolense. A primer pair to a nuclear DNA region incorporated into a separate PCR was employed to control for the presence of amplifiable genomic DNA (representing the..

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University of Melbourne Researchers