Journal article

Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation

Robin B Gasser, Min Hu, Neil B Chilton, Bronwyn E Campbell, Aaron J Jex, Domenico Otranto, Claudia Cafarchia, Ian Beveridge, Xingquan Zhu

NATURE PROTOCOLS | NATURE PUBLISHING GROUP | Published : 2006

Abstract

The accurate analysis of genetic variation has major implications in many areas of biomedical research, including the identification of infectious agents (such as parasites), the diagnosis of infections, and the detection of unknown or known disease-causing mutations. Mutation scanning methods, including PCR-coupled single-strand conformation polymorphism (SSCP), have significant advantages over many other nucleic acid techniques for the accurate analysis of allelic and mutational sequence variation. The present protocol describes the SSCP method of analysis, including all steps from the small-scale isolation of genomic DNA and PCR amplification of target sequences, through to the gel-based ..

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