Journal article

Survival and differentiation of pituitary colony-forming cells in vivo

Diana A Lepore, Gregory PL Thomas, Kenneth R Knight, Alan J Hussey, Thomas Callahan, Josef Wagner, Wayne A Morrison, Paul Q Thomas

STEM CELLS | WILEY | Published : 2007


Growth hormone (GH) deficiency is a significant clinical problem, since growth hormone is essential for the regulation of growth, metabolism, and the cardiovascular system. Stem and progenitor cells have been identified in many adult tissues. Recently, our laboratory identified a cell type within the adult pituitary gland with stem cell-like properties, which we have termed pituitary colony-forming cells (PCFCs). Herein we investigate the ability of PCFCs to survive and differentiate in vivo. Enriched populations of PCFCs were transplanted into an in vivo microchamber model. Grafts were harvested at 6 weeks post-transplant and tested for surviving donor cells (LacZ(+)) or for differentiation..

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