Journal article

A novel flow cytometric phagocytosis assay of malaria-infected erythrocytes

Emma Tippett, Liselle A Fernandes, Stephen J Rogerson, Anthony Jaworowski

JOURNAL OF IMMUNOLOGICAL METHODS | ELSEVIER | Published : 2007

Abstract

Monocytes play a crucial role in controlling malaria infection. To facilitate our research into the development of antibody-mediated immunity against pregnancy-associated malaria we have established several novel malaria-specific flow cytometric phagocytosis assays based on ethidium bromide staining of DNA present in blood stage trophozoites. The first assay quantifies the ability of sera to opsonise trophozoites and promotes phagocytosis by the monocytic cell line THP1. This measures the levels of functional antibodies to the chosen strain of parasite. The second assay is a whole blood phagocytosis assay which measures the phagocytic ability of patient monocytes ex vivo. The third assay emp..

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University of Melbourne Researchers