Journal article

Mycoplasma hyopneumoniae mhp379 is a Ca2 -dependent, sugar-nonspecific exonuclease exposed on the cell surface

Jonathan A Schmidt, Glenn F Browning, Philip F Markham

JOURNAL OF BACTERIOLOGY | AMER SOC MICROBIOLOGY | Published : 2007

Abstract

Mycoplasma hyopneumoniae mhp379 is a putative lipoprotein that shares significant amino acid sequence similarity with a family of bacterial thermostable nucleases. To examine the nuclease activity of mhp379, the gene was cloned and expressed in Escherichia coli following the deletion of the amino-terminal signal sequence and prokaryotic lipoprotein cleavage site and mutagenesis of the mycoplasma TGA tryptophan codons to TGG. The recombinant fusion protein yielded a 33-kDa thrombin cleavage product, corresponding in size to the mature mhp379 protein. Exonuclease activity was indicated by agarose gel electrophoresis analysis of the reaction products that were released when different nucleic ac..

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