Journal article

Aberrant mast-cell differentiation in mice lacking the stem-cell leukemia gene

Jessica M Salmon, Nicholas J Slater, Mark A Hall, Matthew P McCormack, Stephen L Nutt, Stephen M Jane, David J Curtis

BLOOD | AMER SOC HEMATOLOGY | Published : 2007

Abstract

The stem cell leukemia (SCL) gene encodes a basic helix-loop-helix transcription factor expressed in erythroid, megakaryocyte, and mast-cell lineages. SCL is essential for growth of megakaryocyte and erythroid progenitors. We have used a conditional knockout of SCL (SCL(-/Delta)) to examine its function in mast cells, critical effectors of the immune system. SCL(-/Delta) mice had markedly increased numbers of mast-cell progenitors (MCPs) within the peritoneal fluid, bone marrow, and spleen. Fractionation of bone marrow myeloid progenitors demonstrated that these MCPs were present in the megakaryocyte-erythroid-restricted cell fraction. In contrast, unilineage MCPs from control mice were pres..

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