Journal article

Paradoxical effects of IL-12 in leishmaniasis in the presence and absence of vaccinating antigen

AH Noormohammadi, H Hochrein, JM Curtis, TM Baldwin, E Handman

VACCINE | ELSEVIER SCI LTD | Published : 2001


Protective immunity against Leishmania major requires parasite-specific CD4+T helper cells, the development of which is promoted by interleukin 12 (IL-12). In this study we investigated the use of IL-12 DNA to enhance the protective immunity induced by prophylactic vaccination with the L. major Parasite Surface Antigen 2 (PSA-2) DNA. A plasmid was constructed in which the two murine IL-12 subunits p35 and p40 were secreted as a biologically active single chain cytokine. The immunomodulatory effects of this IL-12 DNA were examined by codelivery with PSA-2 DNA in susceptible BALB/c and resistant C3H/He mice and subsequent infection with L. major promastigotes. Surprisingly, administration of I..

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