Journal article

Important role of Nox4 type NADPH oxidase in angiogenic responses in human microvascular endothelial cells in vitro

Srinivasa Raju Datla, Hitesh Peshavariya, Gregory J Dusting, Kalyankar Mahadev, Barry J Goldstein, Fan Jiang

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2007

Abstract

OBJECTIVE: Redox signaling mediated by Nox2-containing NADPH oxidase has been implicated in angiogenic responses both in vitro and in vivo. Because Nox4 type NADPH oxidase is also highly expressed in endothelial cells, we studied the role of Nox4 in angiogenic responses in human endothelial cells in culture. METHODS AND RESULTS: Inhibition of Nox4 expression by small interfering RNA reduced angiogenic responses as assessed by the tube formation and wound healing assays, in both human microvascular and umbilical vein endothelial cells. Overexpression of wild-type Nox4 enhanced, whereas expression of a dominant negative form of Nox4 suppressed the angiogenic responses in endothelial cells. The..

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