Journal article

T84-intestinal epithelial exosomes bear MHC class II/peptide complexes potentiating antigen presentation by dendritic cells

Julia Mallegol, Guillaume Van Niel, Corinne Lebreton, Yves Lepelletier, Celine Candalh, Christophe Dugave, Joan K Heath, Graca Raposo, Nadine Cerf-Bensussan, Martine Heyman

GASTROENTEROLOGY | W B SAUNDERS CO-ELSEVIER INC | Published : 2007

Abstract

BACKGROUND & AIMS: Intestinal epithelial cells release antigen-presenting vesicles (exosomes) bearing major histocompatibility complex class II/peptide complexes stimulating specific immune responses in vivo. To characterize further the role of human epithelial exosomes in antigen presentation, their capacity to load antigenic peptides, bind immune target cells, and induce T-cell activation was analyzed in vitro. METHODS: The capacity of exosomes derived from the HLA-DR4-expressing, intestinal epithelial cell line T84 to load the HLA-DR4-specific peptide (3)H-HSA 64-76 and to activate a HLA-DR4-restricted T-cell hybridoma was tested in the presence or absence of human monocyte-derived dendri..

View full abstract