Journal article

c-Myb is required for progenitor cell homeostasis in colonic crypts

Jordane Malaterre, Marina Carpinelli, Matthias Ernst, Warren Alexander, Michael Cooke, Susan Sutton, Sebastian Dworkin, Joan K Heath, Jon Frampton, Grant McArthur, Hans Clevers, Douglas Hilton, Theo Mantamadiotis, Robert G Ramsay

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2007

Abstract

The colonic crypt is the functional unit of the colon mucosa with a central role in ion and water reabsorption. Under steady-state conditions, the distal colonic crypt harbors a single stem cell at its base that gives rise to highly proliferative progenitor cells that differentiate into columnar, goblet, and endocrine cells. The role of c-Myb in crypt homeostasis has not been elucidated. Here we have studied three genetically distinct hypomorphic c-myb mutant mouse strains, all of which show reduced colonic crypt size. The mutations target the key domains of the transcription factor: the DNA binding, transactivation, and negative regulatory domains. In vivo proliferation and cell cycle marke..

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