Journal article

DNA identification of urban Tanytarsini chironomids (Diptera : Chironomidae)

Melissa E Carew, Vincent Pettigrove, Renee L Cox, Ary A Hoffmann

JOURNAL OF THE NORTH AMERICAN BENTHOLOGICAL SOCIETY | NORTH AMER BENTHOLOGICAL SOC | Published : 2007

Abstract

DNA barcoding is becoming widely used to provide species identifications in a variety of invertebrate taxa, but there has been little application so far to environmental monitoring. Here we make this connection using a group of aquatic macroinvertebrates, chironomids of the tribe Tanytarsini. Tanytarsini larvae commonly collected in biological surveys can be difficult to identify to species because of high intraspecific variability and because not all larvae are linked to described adult life stages. We examined whether Tanytarsini larvae could be reliably identified to species with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using the mitochondrial cytochro..

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