Journal article

Angiogenic growth factor synergism in a murine tissue engineering model of angiogenesis and adipogenesis

John A Rophael, Randall O Craft, Jason A Palmer, Alan J Hussey, Gregory PL Thomas, Wayne A Morrison, Anthony J Penington, Geraldine M Mitchell

AMERICAN JOURNAL OF PATHOLOGY | AMER SOC INVESTIGATIVE PATHOLOGY, INC | Published : 2007

Abstract

De novo tissue generation stimulated by three angiogenic growth factors administered in a factorial design was studied in an in vivo murine tissue engineering chamber. A silicone chamber was implanted around the epigastric pedicle and filled with Matrigel with 100 ng/ml of recombinant mouse vascular endothelial growth factor-120 (VEGF120), recombinant human basic fibroblastic growth factor (FGF-2), or recombinant rat platelet-derived growth factor-BB (PDGF-BB) added as single, double, or triple combinations. Angiogenesis, supporting tissue ingrowth, and adipogenesis were assessed at 2 and 6 weeks by immunohistochemistry and morphometry. At 2 weeks angiogenesis was synergistically enhanced by..

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